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anti hif 2a  (Novus Biologicals)


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    Structured Review

    Novus Biologicals anti hif 2a
    Anti Hif 2a, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 96/100, based on 581 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+hif+2a/HIF-2+alpha%2FEPAS1+Antibody+-+BSA+Free/pm41920011-303-99-104
    Average 96 stars, based on 581 article reviews
    anti hif 2a - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Western Blot:

    Article Title: Astrocytic cystine/glutamate antiporter is a key regulator of erythropoietin expression in the ischemic retina.
    Article Snippet: .. Anti–Hif-2a (NB100122) and anti-tubulin (NB600-936) antibodies were purchased from Novus Biologicals (Centennial, CO, USA) for immunoblot assay. .. PCR primers for TaqMan gene expression assay [human solute carrier family 7, member 11 (Slc7a11): Hs00921938_m1; human EPO (hEPO): Hs01071097_m1; human glyceraldehyde 3- phosphate dehydrogenase (GAPDH): Hs02786624_g1; murine Slc7a11: Mm00442530_m1; murine EPO: Mm01202755_m1; murine GAPDH: Mm99999915_g1)] were purchased from Thermo Fisher Scientific.

    Incubation:

    Article Title: HIF-2α Preserves Mitochondrial Activity and Glucose Sensing in Compensating β-Cells in Obesity.
    Article Snippet: .. Fixed tissue sections were incubated with primary antibodies (guinea pig anti-human insulin [cat. no. IS002; Dako] and rabbit anti–HIF-2a [cat. no. NB100-122; Novus]) (dilution of 1:200), followed by incubation with secondary antibodies. .. For detecting insulin, anti-rabbit IgG (goat) conjugated with biotin (cat. no. NEF813001EA; Jackson ImmunoResearch Laboratories) and streptavidin conjugated with Alexa Fluor 647 (cat. no. 016-600-084; Jackson ImmunoResearch Laboratories) were used.

    Article Title: Decidual protein induced by progesterone enhances HIF-1α stability to promote mitophagy and glycolysis.
    Article Snippet: doi:10.1111/febs.70489 Mitophagy, the process of removing mitochondria through the autophagy– lysosome pathway, is crucial for maintaining cellular homeostasis.. However, its regulatory mechanisms and pathological implications remain poorly understood.. In our study, we revealed that decidual protein induced by progesterone (DEPP) plays a significant role in mitophagy through the DEPP–HIF-1a–BNIP3/NIX axis.

    Article Title: HIF-1α-activated TM4SF1-AS1 promotes the proliferation, migration, and invasion of hepatocellular carcinoma cells by enhancing TM4SF1 expression.
    Article Snippet: Long non-coding RNAs (lncRNAs) are essential drivers or suppressors in human hepatocellular carcinoma (HCC) by participating in controlling transcription, translation, mRNA stability, and protein degradation protein-protein interaction.. TM4SF1-AS1 is recently identified as a tumor-promoting factor in lung cancer.. Nevertheless, its function in HCC and related molecular mechanisms remain unknown.

    Article Title: Palmitate deranges erythropoietin production via transcription factor ATF4 activation of unfolded protein response.
    Article Snippet: .. The membranes were then incubated with rabbit polyclonal anti-HIF-1a, anti-HIF-2a (diluted 1:1000; Novus Biologicals, Littleton, CO), anti-ATF4 antibody, which was raised Kidney International (2018) -, -–- against a peptide mapping near the COOH terminus of ATF4 (diluted 1:200; Santa Cruz Biotechnology, Dallas, TX), or antiGRP78 (diluted 1:200; Santa Cruz Biotechnology), overnight at 4 C. Horseradish peroxidase–conjugated goat anti-mouse antibody (1:10,000; 170–6516; Bio-Rad Laboratories, Hercules, CA), horseradish peroxidase–conjugated goat anti-rabbit IgG antibody (1:10,000; 170–6515; Bio-Rad Laboratories) or horseradish peroxidase–conjugated donkey anti-goat IgG antibody (1:10,000; sc2020; Santa Cruz Biotechnology) was used as secondary antibody. .. Immunoreactive protein was visualized by the chemiluminescence protocol (ECL; GE Healthcare Bio-Sciences).

    Electrochemiluminescence:

    Article Title: Decidual protein induced by progesterone enhances HIF-1α stability to promote mitophagy and glycolysis.
    Article Snippet: doi:10.1111/febs.70489 Mitophagy, the process of removing mitochondria through the autophagy– lysosome pathway, is crucial for maintaining cellular homeostasis.. However, its regulatory mechanisms and pathological implications remain poorly understood.. In our study, we revealed that decidual protein induced by progesterone (DEPP) plays a significant role in mitophagy through the DEPP–HIF-1a–BNIP3/NIX axis.

    Protein Concentration:

    Article Title: Decidual protein induced by progesterone enhances HIF-1α stability to promote mitophagy and glycolysis.
    Article Snippet: doi:10.1111/febs.70489 Mitophagy, the process of removing mitochondria through the autophagy– lysosome pathway, is crucial for maintaining cellular homeostasis.. However, its regulatory mechanisms and pathological implications remain poorly understood.. In our study, we revealed that decidual protein induced by progesterone (DEPP) plays a significant role in mitophagy through the DEPP–HIF-1a–BNIP3/NIX axis.

    Membrane:

    Article Title: HIF-1α-activated TM4SF1-AS1 promotes the proliferation, migration, and invasion of hepatocellular carcinoma cells by enhancing TM4SF1 expression.
    Article Snippet: Long non-coding RNAs (lncRNAs) are essential drivers or suppressors in human hepatocellular carcinoma (HCC) by participating in controlling transcription, translation, mRNA stability, and protein degradation protein-protein interaction.. TM4SF1-AS1 is recently identified as a tumor-promoting factor in lung cancer.. Nevertheless, its function in HCC and related molecular mechanisms remain unknown.



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    The viability of parental PCa cells and the established radioresistant PCa cells 24 h after 5-ALA administration (( a ) PC-3 and PC-3-R; ( b ) DU 145 and DU 145-R). The survival curves of the relative survival fractions in the parental PCa cells and the established radioresistant PCa cells after IR (2–8 Gy single dose) alone or a combination of 1 mM 5-ALA and IR (( c ) PC-3 and PC-3-R; ( d ) DU 145 and DU 145-R). ( e , f ) <t>HIF-1a</t> and <t>HIF-2a</t> expression in the parental and radioresistant PCa cells was measured via Western blot analysis. 5-ALA: 5-aminolevurinic acid. D 50% : radiation dose required to achieve a 50% survival rate. HIF: hypoxia-inducible factor. IC 50 : half-maximal inhibitory concentration. IR: irradiation. PCa: prostate cancer. * p < 0.05, *** p < 0.001. Original western blots are presented in File S1.
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    The viability of parental PCa cells and the established radioresistant PCa cells 24 h after 5-ALA administration (( a ) PC-3 and PC-3-R; ( b ) DU 145 and DU 145-R). The survival curves of the relative survival fractions in the parental PCa cells and the established radioresistant PCa cells after IR (2–8 Gy single dose) alone or a combination of 1 mM 5-ALA and IR (( c ) PC-3 and PC-3-R; ( d ) DU 145 and DU 145-R). ( e , f ) <t>HIF-1a</t> and <t>HIF-2a</t> expression in the parental and radioresistant PCa cells was measured via Western blot analysis. 5-ALA: 5-aminolevurinic acid. D 50% : radiation dose required to achieve a 50% survival rate. HIF: hypoxia-inducible factor. IC 50 : half-maximal inhibitory concentration. IR: irradiation. PCa: prostate cancer. * p < 0.05, *** p < 0.001. Original western blots are presented in File S1.
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    The viability of parental PCa cells and the established radioresistant PCa cells 24 h after 5-ALA administration (( a ) PC-3 and PC-3-R; ( b ) DU 145 and DU 145-R). The survival curves of the relative survival fractions in the parental PCa cells and the established radioresistant PCa cells after IR (2–8 Gy single dose) alone or a combination of 1 mM 5-ALA and IR (( c ) PC-3 and PC-3-R; ( d ) DU 145 and DU 145-R). ( e , f ) <t>HIF-1a</t> and <t>HIF-2a</t> expression in the parental and radioresistant PCa cells was measured via Western blot analysis. 5-ALA: 5-aminolevurinic acid. D 50% : radiation dose required to achieve a 50% survival rate. HIF: hypoxia-inducible factor. IC 50 : half-maximal inhibitory concentration. IR: irradiation. PCa: prostate cancer. * p < 0.05, *** p < 0.001. Original western blots are presented in File S1.
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    The viability of parental PCa cells and the established radioresistant PCa cells 24 h after 5-ALA administration (( a ) PC-3 and PC-3-R; ( b ) DU 145 and DU 145-R). The survival curves of the relative survival fractions in the parental PCa cells and the established radioresistant PCa cells after IR (2–8 Gy single dose) alone or a combination of 1 mM 5-ALA and IR (( c ) PC-3 and PC-3-R; ( d ) DU 145 and DU 145-R). ( e , f ) <t>HIF-1a</t> and <t>HIF-2a</t> expression in the parental and radioresistant PCa cells was measured via Western blot analysis. 5-ALA: 5-aminolevurinic acid. D 50% : radiation dose required to achieve a 50% survival rate. HIF: hypoxia-inducible factor. IC 50 : half-maximal inhibitory concentration. IR: irradiation. PCa: prostate cancer. * p < 0.05, *** p < 0.001. Original western blots are presented in File S1.
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    The viability of parental PCa cells and the established radioresistant PCa cells 24 h after 5-ALA administration (( a ) PC-3 and PC-3-R; ( b ) DU 145 and DU 145-R). The survival curves of the relative survival fractions in the parental PCa cells and the established radioresistant PCa cells after IR (2–8 Gy single dose) alone or a combination of 1 mM 5-ALA and IR (( c ) PC-3 and PC-3-R; ( d ) DU 145 and DU 145-R). ( e , f ) <t>HIF-1a</t> and <t>HIF-2a</t> expression in the parental and radioresistant PCa cells was measured via Western blot analysis. 5-ALA: 5-aminolevurinic acid. D 50% : radiation dose required to achieve a 50% survival rate. HIF: hypoxia-inducible factor. IC 50 : half-maximal inhibitory concentration. IR: irradiation. PCa: prostate cancer. * p < 0.05, *** p < 0.001. Original western blots are presented in File S1.
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    Image Search Results


    The viability of parental PCa cells and the established radioresistant PCa cells 24 h after 5-ALA administration (( a ) PC-3 and PC-3-R; ( b ) DU 145 and DU 145-R). The survival curves of the relative survival fractions in the parental PCa cells and the established radioresistant PCa cells after IR (2–8 Gy single dose) alone or a combination of 1 mM 5-ALA and IR (( c ) PC-3 and PC-3-R; ( d ) DU 145 and DU 145-R). ( e , f ) HIF-1a and HIF-2a expression in the parental and radioresistant PCa cells was measured via Western blot analysis. 5-ALA: 5-aminolevurinic acid. D 50% : radiation dose required to achieve a 50% survival rate. HIF: hypoxia-inducible factor. IC 50 : half-maximal inhibitory concentration. IR: irradiation. PCa: prostate cancer. * p < 0.05, *** p < 0.001. Original western blots are presented in File S1.

    Journal: Cancers

    Article Title: 5-Aminolevulinic Acid: A Novel Approach to Improving Radioresistance in Prostate Cancer

    doi: 10.3390/cancers17081286

    Figure Lengend Snippet: The viability of parental PCa cells and the established radioresistant PCa cells 24 h after 5-ALA administration (( a ) PC-3 and PC-3-R; ( b ) DU 145 and DU 145-R). The survival curves of the relative survival fractions in the parental PCa cells and the established radioresistant PCa cells after IR (2–8 Gy single dose) alone or a combination of 1 mM 5-ALA and IR (( c ) PC-3 and PC-3-R; ( d ) DU 145 and DU 145-R). ( e , f ) HIF-1a and HIF-2a expression in the parental and radioresistant PCa cells was measured via Western blot analysis. 5-ALA: 5-aminolevurinic acid. D 50% : radiation dose required to achieve a 50% survival rate. HIF: hypoxia-inducible factor. IC 50 : half-maximal inhibitory concentration. IR: irradiation. PCa: prostate cancer. * p < 0.05, *** p < 0.001. Original western blots are presented in File S1.

    Article Snippet: The primary antibodies used included anti-β-actin rabbit polyclonal antibody (cat. no. 20536-1-AP; dilution 1:2000; Proteintech, Rosemont, IL, USA), anti-hypoxia-inducible factor 1a (HIF-1a) rabbit polyclonal antibody (cat. No. R12-2180; dilution 1:500; Assay Biotechnology, Fremont, CA, USA), anti-HIF-2a rabbit polyclonal antibody (cat. no. NB100-122; dilution 1:500; Novus Biologicals, Centennial, CO, USA), anti-ATP-binding cassette transporter subfamily G2 (ABCG2) rabbit polyclonal antibody (cat. no. 27286-1-AP; dilution 1:1000; Proteintech), anti-ferrochelatase murine monoclonal antibody (cat. no. sc-377377; dilution 1:500; Santa Cruz Biotechnology, Dallas, TX, USA), anti-proton-coupled peptide transporter 1 (PEPT-1) rabbit polyclonal antibody (cat. no. sc-20653; dilution 1:200; Proteintech), anti-B-cell/CLL lymphoma 2 (BCL-2) murine monoclonal antibody (cat. no. sc-7382; dilution, 1:200; Santa Cruz Bio-technology), anti-BCL-2-associated X protein (BAX) rabbit polyclonal antibody (cat. no. sc-526; dilution, 1:200; Santa Cruz Biotechnology), anti-BCL-2-associated agonist of cell death (BAD) murine monoclonal antibody (cat. no. sc-8044; dilution 1:200; Santa Cruz Biotechnology), and anti-BCL extra-large (BCL-xL) murine monoclonal antibody (cat. no. sc-8392; dilution 1:200; Santa Cruz Biotechnology).

    Techniques: Expressing, Western Blot, Concentration Assay, Irradiation